Cloning and sequencing of the gene encoding chitinase ChiA from Xanthomonas sp. strain AK and some properties of ChiA
| Autor: | Shuichi Karita, Kazuo Sakka, Tetsuya Kimura, Jiraporn Sukhumavasi, Akihiro Kawano, Ryo Kusaka, Kunio Ohmiya |
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| Rok vydání: | 1998 |
| Předmět: |
Signal peptide
biology Structural gene Nucleic acid sequence Fibronectin type III domain Molecular cloning biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology Xanthomonas Biochemistry Chitinase biology.protein medicine Escherichia coli Biotechnology |
| Zdroj: | Journal of Fermentation and Bioengineering. 86:527-533 |
| ISSN: | 0922-338X |
| Popis: | The chiA gene encoding chitinase A was cloned into Escherichia coli from Xanthomonas sp. strain AK and its nucleotide sequence was determined. The structural gene consists of 1788 bp encoding 596 amino acids with a predicted molecular weight of 62,122. The deduced ChiA is a modular enzyme composed of an N-terminal signal peptide and four domains in the following order: a chitin-binding domain, two fibronectin type III domains, and a family 18 catalytic domain. ChiA purified from the recombinant E. coli had temperature and pH optima at 35°C and 4.5, respectively. The Km and Vmax values for colloidal chitin were estimated to be 1.8 mg/ml and 8.7 μmol/min/mg, respectively. |
| Databáze: | OpenAIRE |
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