Development of a reliable and rapid detached leaf assay to detect resistance to the fungal disease phomopsis leaf blight, caused byDiaporthe toxica, inLupinus albus

Autor:	Raymond Cowley, John D. I. Harper, Gavin Ash, David Luckett
Rok vydání:	2012
Předmět:	biology Inoculation fungi food and beverages Plant Science biology.organism_classification Spore Fungal disease Lupinus Phomopsis Diaporthe toxica Botany Blight Cultivar Agronomy and Crop Science
Zdroj:	Canadian Journal of Plant Pathology. 34:401-409
ISSN:	1715-2992 0706-0661
DOI:	10.1080/07060661.2012.705327
Popis:	A detached leaf assay was developed to assess disease response of Lupinus albus to phomopsis leaf blight caused by Diaporthe toxica. A spore concentration of 1 × 107 spores mL−1 using spray inoculation gave the greatest discrimination between genotypes. The effect of leaf position was studied using the first five fully expanded leaves above the cotyledons. Leaf position did not alter the disease response for genotypes with a resistant reaction, but older leaves were more susceptible in less-resistant genotypes. There was good agreement between disease response using detached leaves and leaves inoculated in planta, indicating the utility of the detached leaf assay. In this study, 110 Lupinus albus cultivars were evaluated for resistance to phomopsis leaf blight caused by Diaporthe toxica in two detached leaf experiments. In one experiment, 95 genotypes were assessed for phomopsis leaf blight resistance and phomopsis stem blight resistance after the leaves used in this study were removed. There was...
Databáze:	OpenAIRE
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