Identification of a P2-related prophage remnant locus ofPhotorhabdus luminescensencoding an R-type phage tail-like particle

Autor:	Noël Boemare, Eric Duchaud, Sophie Gaudriault, Frank Kunst, Jacques-Olivier Thaler, Alain Givaudan
Rok vydání:	2004
Předmět:	Genetics biology Locus (genetics) biology.organism_classification Microbiology Molecular biology Bacteriophage Photorhabdus luminescens Lysogenic cycle Bacteriophage P2 Molecular Biology Gene Photorhabdus Prophage
Zdroj:	FEMS Microbiology Letters. 233:223-231
ISSN:	1574-6968 0378-1097
Popis:	Analysis of the Photorhabdus luminescens genome sequence revealed that the pts region is related to the tail synthesis gene core of the P2 phage. The pts locus encodes a DNA invertase homologue. PCR-RFLP analysis showed the two potential tail fiber regions of the pts locus present DNA inversions. Electron microscopy revealed a phage tail-like particle, related to the R-type family and named R-photorhabdicin, in the culture supernatant of P. luminescens. Mass spectrometry analysis of two sub-units of R-photorhabdicin revealed that they are encoded by the pts locus. The role of this P2-related prophage remnant in the Photorhabdus genome is discussed.
Databáze:	OpenAIRE
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