Effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb
| Autor: | L. N. Mironova, E. V. Boltenkov, E. V. Zarembo |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
fungi food and beverages Biology biology.organism_classification General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Basal shoot Murashige and Skoog medium chemistry Auxin Callus Shoot Botany Primordium Iris ensata Kinetin General Agricultural and Biological Sciences |
| Zdroj: | Biology Bulletin. 34:446-450 |
| ISSN: | 1608-3059 1062-3590 |
| DOI: | 10.1134/s1062359007050044 |
| Popis: | The effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb. was studied. 2,4-Dichlorophenoxyacetic acid (2,4-D) proved to be the most suitable auxin for the induction and subculturing of morphogenic callus. Organogenic calluses were induced from isolated embryos at the waxphase in MS medium supplemented with 2,4-D (1.0–2.0 mg/l) and kinetin (0.2–0.5 mg/l). Changes in the medium hormonal composition favor the development of adventitious structures. The setting of adventitious shoot buds took place in the presence of 6-benzylaminopurine, while the development of shoots and root primordia was observed after 2,4-D replacement with indoleacetic acid (2.0 mg/l). The root initiation in regenerating plants required a hormone-free medium. The development of intact seedling and regenerated plants of I. ensata were studied. Analysis of the shoot structure demonstrated that in vitro cultured plants are at the juvenile stage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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