Regeneration and retention of NADP (H) for xylitol production in an ionized membrane reactor

Autor:	Vichien Kitpreechavanich, Naomichi Nishio, Mitsunori Hayashi, Shiro Nagai
Rok vydání:	1985
Předmět:	chemistry.chemical_classification Chromatography biology Membrane reactor Bioengineering General Medicine Xylose Permeation Xylitol Applied Microbiology and Biotechnology Cofactor chemistry.chemical_compound Membrane chemistry Oxidoreductase Yield (chemistry) biology.protein Biotechnology
Zdroj:	Biotechnology Letters. 7:657-662
ISSN:	1573-6776 0141-5492
Popis:	A sulfonated polysulfone membrane reactor was used forin situ regeneration and retention of coenzymes NADP (H) using the xylose reductase ofCandida pelliculosa coupled with oxidoreductase system ofMethanobacterium sp. in the reduction of xylose to xylitol with hydrogen gas. The membrane could almost completely reject the permeation of NADP (H) (92 and 97%), F420 (97%) and the required enzymes (100%), but not reject for the permeation of xylitol (product). After 4-h reaction for the production of xylitol from xylose (93% yield), although 25% NADP (H) initially added was lost its activity due to unavoidable degradation, the membrane could reject the permeation of the remaining NADP (H) and F420 at the level of 90 and 95%, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::45cd8c26efb0ddbc1c2f72dba366592f https://doi.org/10.1007/bf01040204 Zobrazit plný text záznamu Full text from SpringerLink