Bacillus megaterium als Produktionssystem für rekombinante Proteine
| Autor: | Rebekka Biedendieck, Rajan Hollmann, Marco Malten, Yang Yang, Dieter Jahn, Wei Wang, Wolf-Dieter Deckwer |
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| Rok vydání: | 2006 |
| Předmět: |
Cloning
biology Chemistry General Chemical Engineering fungi Promoter General Chemistry biology.organism_classification medicine.disease_cause Industrial and Manufacturing Engineering law.invention Secretory protein Plasmid Biochemistry law Gene expression Recombinant DNA medicine bacteria Escherichia coli Bacillus megaterium |
| Zdroj: | Chemie Ingenieur Technik. 78:289-294 |
| ISSN: | 1522-2640 0009-286X |
| Popis: | During the past years plasmid systems for recombinant protein production using Bacillus megaterium were developed. These systems are based on multicopy plasmids with functional elements for cloning in Escherichia coli and induced gene expression in B. megaterium. Different promoters, signals for protein secretion and affinity tags for purification arranged in a high variety of combinations are available. By using these systems, recombinant proteins can be produced and secreted in the grams per litre scale. |
| Databáze: | OpenAIRE |
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