Expression of Intracellular Th1 and Th2 Cytokines in Women with Recurrent Spontaneous Abortion, Implantation Failures after IVF/ET or Normal Pregnancy
| Autor: | Poonam Thaker, Siu Chui Ng, Alan E. Beer, Alice Gilman-Sachs, Joanne Kwak-Kim, Kenneth D. Beaman |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Pregnancy business.industry medicine.medical_treatment Immunology Obstetrics and Gynecology Interleukin medicine.disease Embryo transfer Cytokine Endocrinology Reproductive Medicine Internal medicine Immunology and Allergy Medicine Gestation Interferon gamma business Interleukin 4 CD8 medicine.drug |
| Zdroj: | American Journal of Reproductive Immunology. 48:77-86 |
| ISSN: | 1046-7408 |
| DOI: | 10.1034/j.1600-0897.2002.01105.x |
| Popis: | PROBLEM: We aimed to investigate absolute counts of intracellular T helper 1 (Th1) and Th2 cytokine expressing T-cell subpopulations in women with three or more recurrent spontaneous abortions (RSA), multiple implantation failures after in-vitro fertilization and embryo transfer (IVF/ET) (three or more) or during normal pregnancy. METHOD OF STUDY: Absolute cell counts and percentages of CD3+, CD3+/ CD4+, and CD3+/CD8+ T-cell populations expressing intracellular cytokines [interferon-gamma (IFN-y), tumor necrosis factor-alpha (TNF-α), interleukin (IL)-4 and IL-10] was studied by four-color flow cytometry in 15 RSA and 13 implantation failure patients. Eighteen fertile non-pregnant and 47 normal pregnant women were also compared with regard to intracellular cytokine expression. RESULTS: Interleukin-10 producing CD3 + /CD8+ T-cell counts were significantly lower in women with RSA (P < 0.05) and implantation failures (P < 0.05), and TNF-a producing CD3+/CD4+ T-cell counts were higher in women with RSA (P < 0.05) and implantation failures (P < 0.005) than those of non-pregnant fertile controls. During normal pregnancies, first trimester IL-4 expressing CD3 +, CD3 +/ CD4+ T-cell counts (P < 0.05) and IFN-y expressing CD3+ T-cell counts (P < 0.05) were significantly higher than those of third trimester (P < 0.05). First trimester TNF-α expressing CD3+/CD8+ T-cell counts were significantly higher than those of second and third trimester women (P < 0.05). However, there are no differences in cytokine expression between non-pregnant and first trimester pregnant women. CONCLUSION: Absolute counts of IFN-y, IL-4, and TNF-a expressing T cells decrease with the progress of gestation (third trimester) during normal pregnancies. In women with implantation failures, absolute cell counts of TNF-α expressing CD3 + / 4+ cells reflects the presence of dominant Th1 immune response. A significantly increased Th1 cytokine expression may be the underlying immune etiology for reproductive failures. |
| Databáze: | OpenAIRE |
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