Hsa-miR-202-3p, up-regulated in type 1 gastric neuroendocrine neoplasms, may targetDUSP1
| Autor: | Ying-Ying Liu, Dou Dou, Yanfen Shi, Qing Liu, Yuan-liang Li, Meng Liu, Huangying Tan, Jie Luo, Jixi Liu, Xu-Dong Qiu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Reporter gene HEK 293 cells Phosphatase Gastroenterology General Medicine Biology Pathogenesis 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Downregulation and upregulation Mirna expression 030220 oncology & carcinogenesis microRNA Cancer research Gene |
| Zdroj: | World Journal of Gastroenterology. 24:573-582 |
| ISSN: | 1007-9327 |
| Popis: | Aim To detect abnormal microRNA (miRNA) expression in type 1 gastric neuroendocrine neoplasms (g-NENs) and find potential target genes. Methods Tumour tissues from patients with type 1 g-NENs were used as experimental samples, and gastric mucosal tissues from the same patients obtained during gastroscopy review after several months were used as control samples. miRNA expression was examined with Agilent human miRNA chips and validated via RT-PCR. Three types of target gene prediction software (TargetScan, PITA, and microRNAorg) were used to predict potential target genes of the differentially expressed miRNAs, and a dual-luciferase reporter assay system was used for verification. Results Six miRNAs were significantly upregulated or downregulated in the tumours compared to the control samples. Among them, miR-202-3p was extraordinarily upregulated. RT-PCR of seven sample sets confirmed that miR-202-3p was upregulated in tumour tissues. In total, 215 target genes were predicted to be associated with miR-202-3p. Among them, dual-specificity phosphatase 1 (DUSP1) was reported to be closely related to tumour occurrence and development. The dual-luciferase reporter assay showed that miR-202-3p directly regulated DUSP1 in 293T cells. Conclusion miR-202-3p is upregulated in type 1 g-NEN lesions and might play important roles in the pathogenesis of type 1 g-NENs by targeting DUSP1. |
| Databáze: | OpenAIRE |
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