Complex Formation of Whey Proteins: Exocellular Polysaccharide EPS B40
| Autor: | Fanny Weinbreck, Gerard W. Robijn, Cornelis G. de Kruif, Hans Nieuwenhuijse |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Chemistry Surfaces and Interfaces Condensed Matter Physics Polysaccharide Light scattering Polyelectrolyte Viscosity Crystallography Isoelectric point Chemical engineering Ionic strength Electrochemistry General Materials Science Static light scattering Turbidity Spectroscopy |
| Zdroj: | Langmuir. 19:9404-9410 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Whey proteins (WPs) and the exopolysaccharide B40 (EPS B40) form electrostatic complexes under specific conditions. EPS B40 is a natural thickener in yogurt-like products. It is a phosphated polysaccharide and thus has a strong polyelectrolyte character. When the WP and the EPS B40 were mixed at pH values near or below the isoelectric point (pI) of the protein, soluble complexes were formed at pHc and phase separation took place below pHφ. The formation and the structure of those complexes were studied by various methods, including turbidity, dynamic and static light scattering (DLS and SLS), and viscosity measurements. The results showed that the strength of the interaction was strongly pH- and salt-dependent. The ζ-potential of the protein at pHc and pHφ was linearly dependent on the square root of the ionic strength (√I), showing the electrostatic nature of the interaction. Light scattering and viscosity measurements provided new results on the behavior of the complexes at the molecular level. In the r... |
| Databáze: | OpenAIRE |
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