Development of a capillary electrophoresis system coupled to sequential injection analysis and evaluation by the analysis of nitrophenols
| Autor: | Burkhard Horstkotte, Víctor Cerdà Martín, Olaf Elsholz |
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| Rok vydání: | 2007 |
| Předmět: |
Reproducibility
Chromatography Sequential injection analysis Chemistry Capillary action Health Toxicology and Mutagenesis Instrumentation aspects Public Health Environmental and Occupational Health Analytical chemistry Soil Science Pollution Analytical Chemistry Capillary electrophoresis Light source Calibration Environmental Chemistry Solenoid valve Waste Management and Disposal Water Science and Technology |
| Zdroj: | International Journal of Environmental Analytical Chemistry. 87:797-811 |
| ISSN: | 1029-0397 0306-7319 |
| DOI: | 10.1080/03067310701272863 |
| Popis: | A combination of a laboratory-made capillary electrophoresis system and a sequential injection analysis equipment is described. For characterization, the system was successfully applied to the separation and quantification of nitrophenols. A blue LED was used as light source, and hydrodynamic injection was carried out by using a pressure-stable solenoid valve and an inflatable pressure reservoir. A good reproducibility of migration time (0.5%) and peak heights (5%) were obtained. The calibration by using peak heights was found to be linear up to 776 µmol L−1 for all three compounds. The system was robust and reliable for autonomous analysis without observation. All maintenance requirements including the conditioning of the capillary and flushing of both buffer reservoirs were carried out automatically. Instrumentation aspects of the capillary electrophoresis part are compared with former described hyphenated flow systems showing maximal operation versatility. Instrumental control and data evaluation were ... |
| Databáze: | OpenAIRE |
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