Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation
| Autor: | Aikaterina Siopi, Vassilis Mougios |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Test meal medicine.medical_specialty business.industry Parallel design 010401 analytical chemistry Confounding 01 natural sciences Crossover study 0104 chemical sciences 03 medical and health sciences 030104 developmental biology Metabolomics Physical medicine and rehabilitation Metabolome Medicine Exercise metabolism business Biological variability |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493976423 |
| DOI: | 10.1007/978-1-4939-7643-0_19 |
| Popis: | Metabolomics can be of great value in the study of exercise metabolism. However, because of the high intraindividual and interindividual biological variability of the human metabolome, special considerations should be taken into account when designing an acute-exercise metabolomic study. To study different exercise parameters, e.g., different exercise modes, intensities, etc., a crossover study design, where each participant acts as their own control, is preferable to a parallel design, one involving different groups of participants. Moreover, the study should include a no exercise, control trial. Before each trial, participants should follow carefully designed preparatory steps to control for possible confounding factors, i.e., maintain repeatable and constant conditions for all individual trials of the study to minimize variation due to factors other than the one(s) being studied. This chapter focuses on the design of human metabolomic studies, where the intervention is an acute metabolic challenge, such as an exercise bout or a test meal, and presents some basic steps for screening potential participants, performing preliminary tests, preparing for the trial day, and performing the trial. |
| Databáze: | OpenAIRE |
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