Quantitative phase imaging of cells through turbid media based on infrared digital holographic microscopy

Autor:	Xiaodong Hu, Yujian Hong, Junsheng Lu, Xinyu Chang, Yanan Zeng
Rok vydání:	2021
Předmět:	Materials science Optics Quantitative phase microscopy Optical imaging business.industry Infrared Phase imaging Near-infrared spectroscopy General Engineering General Physics and Astronomy Digital holographic microscopy business
Zdroj:	Applied Physics Express. 14:035004
ISSN:	1882-0786 1882-0778
DOI:	10.35848/1882-0786/abe601
Popis:	Most biological cells live in turbid media that poses an obstacle to real-time measurement of morphology, which is significant in the biomedical field. Imaging through turbid environments is experimentally challenging using visible-light illumination for multiple scattering. Therefore, an infrared digital holographic method is reported herein to test biological samples through turbid media that can achieve real-time three-dimensional morphology measurement. Pure milk is employed as turbid media with 500 μm maximum thickness. The correctness of phase reconstructed from the infrared system is proved compared to that of a visible-light system with less than a 10% deviation of phase difference.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::45787697af91de69215111433653fe0e https://doi.org/10.35848/1882-0786/abe601 Zobrazit plný text záznamu