Bioconversion of acrylonitrile using nitrile hydratase activity of Bacillus sp. APB-6
| Autor: | Duni Chand, Rajendra Singh, Deepak Pandey, Shilpa Dhariwal, Priyanka Sood |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Nitrile Bioconversion 010501 environmental sciences Environmental Science (miscellaneous) Nitrile hydratase activity 01 natural sciences Agricultural and Biological Sciences (miscellaneous) chemistry.chemical_compound chemistry Nitrile hydratase 010608 biotechnology Acrylamide Urea Yeast extract Acrylonitrile 0105 earth and related environmental sciences Biotechnology Nuclear chemistry |
| Zdroj: | 3 Biotech. 8 |
| ISSN: | 2190-5738 2190-572X |
| DOI: | 10.1007/s13205-018-1207-1 |
| Popis: | Bacillus sp. APB-6 harboring nitrile hydratase was used in the production of acrylamide from acrylonitrile. Bacillus sp. APB-6, for maximum production of Co++ containing nitrile hydratase, was cultured in the medium containing lactose (18.0 g l−1), peptone (1.0 g l−1), yeast extract (2.0 g l−1), MgSO4 (0. 5 g l−1), K2HPO4 (0.6 g l−1), urea (9.0 g l−1), and CoCl2 (0.01 g l−1), pH 7.0, and incubated at 35 °C for 24 h in an incubator shaker (160 rpm). Nitrile hydratase exhibited relatively high specificity for aliphatic nitriles. Free cells were immobilized using 2% (w/v) agar solution to enhance enzyme stability and reusability in repetitive cycles of acrylamide production. Under optimized conditions, nearly complete bioconversion of acrylonitrile was achieved with a fair recovery of 85% using free and immobilized cells equivalent to 500 mg dcw l−1. An efficient nitrile hydratase-mediated bioconversion of acrylonitrile to acrylamide at 1-l scale was achieved with time and space productivity of 426 g h−1 g−1 dcw using free cells. |
| Databáze: | OpenAIRE |
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