| Autor: | Valery M. Lipkin, T. A. Zargarova, Natalia Malkova, S. B. Krasnova, Elena V. Navolotskaya, T. N. Lepikhova |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
endocrine system medicine.medical_specialty biology Enkephalin Chemistry Antagonist Peptide General Medicine (+)-Naloxone Biochemistry Endocrinology Opioid Concanavalin A Internal medicine medicine biology.protein Bioorganic chemistry Receptor hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Biochemistry (Moscow). 67:357-363 |
| ISSN: | 0006-2979 |
| DOI: | 10.1023/a:1014844718799 |
| Popis: | β-Endorphin and the synthetic β-endorphin-like decapeptide Ser-Leu-Thr-Cys-Leu-Val-Lys-Gly-Phe-Tyr (referred to as immunorphin), corresponding to the sequence 364-373 of the CH3 domain of human immunoglobulin G heavy chain, were shown to stimulate concanavalin A-induced proliferation of T lymphocytes from the blood of healthy donors. [Met5]Enkephalin and the antagonist of opioid receptors naloxone examined in parallel were inactive. The stimulating effect of β-endorphin and immunorphin on T lymphocyte proliferation is not inhibited by naloxone. Studies on receptor binding of 125I-labeled immunorphin to T lymphocytes revealed that it binds with high affinity to naloxone-insensitive receptors (K d = 7.0 ± 0.3 nM)). Unlabeled immunorphin completely inhibits 125I-labeled β-endorphin specific binding to naloxone insensitive receptors on T lymphocytes (K i = 0.6 ± 0.1 nM)). Thus, β-endorphin and immunorphin interact with common naloxone insensitive receptors on T lymphocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink