The striking effects of spacer and terminator sequences on the synthesis of porcine growth hormone inEscherichia coli

Autor:	Lai Ling Soong, Shih Tung Liu, Wen Chang Chang, Yuh Shan Jou, Huei Ming Lin, Bing Fung Chen, Lih Hwa S. Hwang
Rok vydání:	1990
Předmět:	Expression vector Nucleic acid sequence Bioengineering Promoter General Medicine Biology medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology Terminator (genetics) Start codon Gene expression Consensus sequence medicine Escherichia coli Biotechnology
Zdroj:	Biotechnology Letters. 12:335-340
ISSN:	1573-6776 0141-5492
DOI:	10.1007/bf01024427
Popis:	Two types of expression vectors containing theP tac orP L promoters, synthetic ribosomalbinding sequences (RBS), and a transciptional terminator (rrnBT1T2) were constructed for the production of porcine growth hormone (pGH) inEscherichia coli. The results demonstrated that the nucleotide sequence between the RBS and the start codon, ATG, was critical for the yields of pGH. The rrnBT1T2 terminator was essential for the efficient expression ofpGH in theP tac -driven vectors, but reduced the synthesis of pGH in theP L -vectors. A leaky repression was observed in the JM103 cells containing theP tac -plasmids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4568e0930f59fff242c0f01416a1fcf3 https://doi.org/10.1007/bf01024427 Zobrazit plný text záznamu Full text from SpringerLink