Evidence of superoxide dismutase in Lactobacillus acidophilus
| Autor: | Ana M. Strasser de Saad, Guillermo Oliver, Silvia N. González, María C. Apella, Carlos A. Nadra Chaud |
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| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
biology Size-exclusion chromatography food and beverages General Chemistry General Medicine Lactobacillaceae biology.organism_classification Enzyme assay Superoxide dismutase Enzyme Lactobacillus acidophilus chemistry Biochemistry Drug Discovery biology.protein Specific activity Polyacrylamide gel electrophoresis |
| Zdroj: | Chemical and Pharmaceutical Bulletin. 39:1065-1067 |
| ISSN: | 1347-5223 0009-2363 |
| DOI: | 10.1248/cpb.39.1065 |
| Popis: | Superoxide dismutase was prepared from Lactobacillus acidophilus CRL 358. An approximately 191-fold increase in the specific activity of the enzyme was achieved. The extract purified to apparent homogenity and exhibited a single band following polyacrylamide gel electrophoresis which detected superoxide dismutase activity. The apparent molecular weight determined by filtration was about 160000 dalton (Da). |
| Databáze: | OpenAIRE |
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