The importance of yeast Alr proteins in cadmium detoxification as indicated by particle-induced x-ray emission and phenotypic analyses

Autor:	Johnny Ferraz Dias, Maria Lucia Yoneama, Diego Bonatto, Martin Brendel, Ana Lúcia Kern, João Antonio Pêgas Henriques
Rok vydání:	2005
Předmět:	Cadmium biology Mutant Saccharomyces cerevisiae Analytical chemistry chemistry.chemical_element Particle-induced X-ray emission biology.organism_classification Transmembrane protein Yeast Metal chemistry visual_art visual_art.visual_art_medium Biophysics Spectroscopy Intracellular
Zdroj:	X-Ray Spectrometry. 34:355-358
ISSN:	1097-4539 0049-8246
DOI:	10.1002/xrs.835
Popis:	One gene in Saccharomyces cerevisiae, ALR1, encodes a transmembrane protein involved in the transport of Mg 2+ into cells. Overexpression of this gene changes the tolerance of S. cerevisiae to several metal cations. In order to study the cellular uptake mechanism of metal cations in S. cerevisiae, an accurate determination of the metals present in the cells is needed. In this work, particle-induced x-ray emission (PIXE) was employed to analyze the intracellular metal content after cell exposure to cadmium. To that end, a specific method for sample preparation was developed. The samples were irradiated with a 2 MeV proton beam, while the induced x-rays were detected with a high-purity germanium detector. The PIXE results for cadmium-exposed ALR1 mutant and wild-type yeast cells suggest that Alr1p has a central role in the cell survival process in a cadmium-rich environment.
Databáze:	OpenAIRE
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