Isolation and characterisation of a cold-inducedDREBgene fromCymbidium insigne
| Autor: | Rui Bai, Cong-Fen He, Na Yao, Hua Zhao, Lu-Bin Li, Tao Wang, Lei Liu |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | The Journal of Horticultural Science and Biotechnology. 86:43-49 |
| ISSN: | 2380-4084 1462-0316 |
| DOI: | 10.1080/14620316.2011.11512723 |
| Popis: | SummaryDehydration-responsive element DNA binding (DREB) proteins mediate responses to several environmental stress in plants. A novel CiDREB1 gene of 1,265 bp was isolated from Cymbidium insigne by RACE. The deduced amino acid sequence had a predicted molecular mass of 34.43 kDa and a pI of 5.20. A BLAST search revealed that the encoded protein could be classified as a member of the AP2/EREBP family. Expression analysis showed that CiDREB1 gene transcripts accumulated rapidly under cold stress and peaked by 6 h. Yeast one-hybrid analysis indicated that CiDREB1 could interact with its DNA binding domain to activate expression of the dual reporter genes histidine (His3) and galactosidase (LacZ). CiDREB1 localised to the nucleus, although it lacked the typical nuclear localisation signal (NLS). We inserted the CiDREB1 gene into the binary transformation vector, pBI121 which was then transferred into tobacco plants using Agrobacterium. Forty-seven transgenic tobacco lines were identified by kanamycin and car... |
| Databáze: | OpenAIRE |
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