Real-time quantification of TEL–AML1 fusion transcripts for MRD detection in relapsed childhood acute lymphoblastic leukaemia
| Autor: | Tillmann Taube, Günter Henze, Karlheinz Seeger, Cornelia Eckert, Gabriele Körner |
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| Rok vydání: | 2004 |
| Předmět: |
Cancer Research
Quantification methods Response to therapy business.industry Hematology Minimal residual disease body regions Quantitative Real Time PCR Oncology Fusion transcript hemic and lymphatic diseases Antigen receptor Immunology Tel aml1 Cancer research Medicine Lymphoblastic leukaemia business neoplasms |
| Zdroj: | Leukemia Research. 28:699-706 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/j.leukres.2003.11.006 |
| Popis: | Response to therapy of ALL assessed by molecular methods has been proved to be a predictor of outcome. Alternatively to established but very labour-intensive DNA-based PCR-techniques the TEL-AML1 fusion transcript can serve as a marker for MRD monitoring. MRD quantification using TEL-AML1 is of particular interest if the results are directly comparable to data obtained by established DNA-based assays. Investigation of the potential of MRD monitoring using LightCycler technology for TEL-AML1 real-time quantification and comparison to results from established DNA-based MRD assays revealed corresponding results. Accordingly, TEL-AML1 MRD quantification is a sensitive, specific and rapid method that can supplement clone-specific MRD detection. |
| Databáze: | OpenAIRE |
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