HLA-B*51 allele analysis by the PCR-SBT method and a strong association of HLA-B*5101 with Japanese patients with Behçet’s disease
| Autor: | Yoshihiko Katsuyama, Masao Ota, Hidetoshi Inoko, Katsuhiro Onari, Takashi Shiina, H. Ando, Kazuro Yabuki, Nobuhisa Mizuki, E. Nomura, Shigeaki Ohno |
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| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Incidence (epidemiology) Immunology General Medicine Behcet's disease Human leukocyte antigen medicine.disease Biochemistry eye diseases HLA-B stomatognathic diseases Antigen Genetics Immunology and Allergy Medicine Typing Allele business Genotyping |
| Zdroj: | Tissue Antigens. 58:181-184 |
| ISSN: | 0001-2815 0000-0000 |
| DOI: | 10.1034/j.1399-0039.2001.580306.x |
| Popis: | Behcet’s disease (BD) is known to be associated with human leukocyte antigen (HLA) B51 in many different ethnic groups. An increased incidence of HLA-B51 in the patient group has also been reported in a Japanese population. Recently, the B51 antigen has been identified to comprise 21 alleles, B*5101–B*5121. Further, not only HLA-B*5101 but also HLA-B*5108 were found to be relatively increased in the patient groups among Italian and Saudi Arabian populations. Therefore, we performed HLA-B*51 allele genotyping by the polymerase chain reaction-sequencing based typing (PCR-SBT) method in order to investigate whether there is any correlation of one particular B51-associated allele with Japanese BD. Ninety-six Japanese patients with BD and 132 healthy Japanese volunteers were enrolled in this study. As a result, the phenotype frequency of the B51 antigen was confirmed to be remarkably increased in the patient group as compared to the ethnically matched control group (59.4% in patients vs. 13.6% in controls; Pc=0.0000000000098, R.R.=9.3). In the B*51 allele genotyping, 56 out of 57 B51-positive patients were defined as B*5101 and the remaining one was B*5102. In contrast, all of 18 B51-positive normal controls were B*5101. None of the Japanese patients and healthy controls carried the HLA-B*5108 allele. This study revealed that B*51 allelic distribution in Japanese was different from those in Italian and Saudi Arabian populations, and that the significantly high incidence of the HLA-B51 antigen in the Japanese BD patient group was mostly caused by the significant increase of the HLA-B*5101 allele. |
| Databáze: | OpenAIRE |
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