Detection, Identification and Phylogenetic Analysis of IndianRalstonia solanacearumIsolates byComparison of PartialhrpB Gene Sequences
| Autor: | Bhadvelu Chandrasekhar, Sharanaiah Umesha |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Ralstonia solanacearum
Phylogenetic tree Physiology Bacterial wilt food and beverages Plant Science biochemical phenomena metabolism and nutrition Biology biology.organism_classification Homology (biology) Microbiology law.invention genomic DNA law Genetics Evolutionary divergence Agronomy and Crop Science Gene Polymerase chain reaction |
| Zdroj: | Journal of Phytopathology. 163:105-115 |
| ISSN: | 0931-1785 |
| DOI: | 10.1111/jph.12287 |
| Popis: | A species-specific Polymerase Chain Reaction (sPCR) method was developed to identify and detect isolates of Ralstonia solanacearum, the cause of bacterial wilt disease in chilli. PCR primers for R. solanacearum were identified by alignment of hrpB gene sequences and selection of sequences specific for R. solanacearum at their 3′ ends. The primers were shown to be specific for R. solanacearum, as no PCR product was obtained when genomic DNA from other bacterial species including closely related Ralstonia species, were used as test species. Lone pair of primers (RshrpBF and RshrpBR) was designed using hrpB gene sequence, unique to R. solanacearum which amplified a predicted PCR product of 810 bp from 20 different isolates. Phylogenetic analysis was also attempted to understand the evolutionary divergence of Indian R. solanacearum isolates. Based on phylogenetic analysis, Indian isolates showed homology with the standard reference isolates from other countries but, interestingly, one new isolate showed complete evolutionary divergence by forming an out-group. |
| Databáze: | OpenAIRE |
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