Full-length genome analysis of an avian influenza A virus (H9N2) from a human infection in Changsha City

Autor:	Yuan Jie, Liu Xiaolei, Li Lingzhi, Huang Zheng, Yang Rengui, Ou Xinhua, Yao Dong, Song Yingchun
Rok vydání:	2018
Předmět:	0301 basic medicine Avian Influenza A Virus 03 medical and health sciences 030104 developmental biology viruses Virology 030106 microbiology Biology Full length genome
Zdroj:	Future Virology. 13:323-330
ISSN:	1746-0808 1746-0794
DOI:	10.2217/fvl-2017-0151
Popis:	Aim: Sequencing and phylogenetic analysis of avian influenza A (H9N2) virus strain, which was isolated from a 2-year-old child presenting influenza-like symptoms. Methods: Viral genome was acquired by RT-PCR, and phylogenetic trees were constructed using the neighbor-joining method. Results: A/Hunan/44557/2015 sequence shared identity with the RSSRGLF motif – first reported in low-pathogenic avian influenza in birds. Polymerase L336M and hemagglutinin Q226L mutation was found in the strain. Two newly mutation sites, T197D and D483N in hemagglutinin gene, were found. Phylogenetic tree analysis revealed that the eight gene segments of this virus contained three lineages. Conclusion: A/Hunan/44557/2015 may have acquired the ability to infect humans via genetic exchange with other H9N2 viruses, indicating that the H9N2 genome can generate pandemic isolates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::44df6fc47c87d40677c6a35bb6dae5fa https://doi.org/10.2217/fvl-2017-0151 Zobrazit plný text záznamu