Inhibition of cardiac pacemaker channel hHCN2 depends on intercalation of lipopolysaccharide into channel-containing membrane microdomains
| Autor: | Udo Klöckner, Harald Loppnow, Karl Werdan, Claudia Grossmann, Michael Gekle, Ursula Müller-Werdan, Uwe Rueckschloss, Katja Schumann, Henning Ebelt, Saskia Matzat |
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| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharide
Physiology Sinoatrial node medicine.medical_treatment Beta-Cyclodextrins Biology Potassium channel Cardiac pacemaker Cell biology Lipid A Pacemaker potential chemistry.chemical_compound medicine.anatomical_structure chemistry Immunology medicine lipids (amino acids peptides and proteins) Patch clamp |
| Zdroj: | The Journal of Physiology. 592:1199-1211 |
| ISSN: | 0022-3751 |
| DOI: | 10.1113/jphysiol.2013.268540 |
| Popis: | Depressed heart rate variability in severe inflammatory diseases can be partially explained by the lipopolysaccharide (LPS)-dependent modulation of cardiac pacemaker channels. Recently, we showed that LPS inhibits pacemaker current in sinoatrial node cells and in HEK293 cells expressing cloned pacemaker channels, respectively. The present study was designed to verify whether this inhibition involves LPS-dependent intracellular signalling and to identify structures of LPS responsible for pacemaker current modulation. We examined the effect of LPS on the activity of human hyperpolarization-activated cyclic nucleotide-gated channel 2 (hHCN2) stably expressed in HEK293 cells. In whole-cell recordings, bath application of LPS decreased pacemaker current (IhHCN2) amplitude. The same protocol had no effect on channel activity in cell-attached patch recordings, in which channels are protected from the LPS-containing bath solution. This demonstrates that LPS must interact directly with or close to the channel protein. After cleavage of LPS into lipid A and the polysaccharide chain, neither of them alone impaired IhHCN2, which suggests that modulation of channel activity critically depends on the integrity of the entire LPS molecule. We furthermore showed that β-cyclodextrin interfered with LPS-dependent channel modulation predominantly via scavenging of lipid A, thereby abrogating the capability of LPS to intercalate into target cell membranes. We conclude that LPS impairs IhHCN2 by a local mechanism that is restricted to the vicinity of the channels. Furthermore, intercalation of lipid A into target cell membranes is a prerequisite for the inhibition that is suggested to depend on the direct interaction of the LPS polysaccharide chain with cardiac pacemaker channels. |
| Databáze: | OpenAIRE |
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