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Background Many multiple anti cancer drugs regimens have been established for metastaticcolorectal cancer recently. We investigated cellular immunoreaction of these patients to their cancer. Methods Twenty-six metastatic colorectal cancer patients have been started chemotherapies. Their PBMCwere harvested and investigated their character by Fac scan with fluorescent labeled antibodies (CD3, CD8, CD4, CD25, Foxp3) at before and after chemotherapy. Results After chemotherapy, both CTLs (CD3, CD8 positive) and regulatory T cells (CD4, CD25, Foxp3 positive) were decreased in numberamong all patients. On the other hand, CTL/T reg ratio were significantly increased among tumormarker decreased patients and significantly decreased among tumor marker increased or stablepatients. CEA levels among 85.7% of increased CTL/T reg ratio patients became to decrease less thanhalf. CEA levels among 66.7% of decreased CTL/T reg ratio patients became to increase or to bestable, if their regimens have not been changed. The Reactive Rate of chemotherapy of CTL/T reg ratioincreased patients was significantly higher than that of ratio decreased patients. (P = 0.021) The Disease Control Rate of chemotherapy of CTL/T reg ratio increased patients was higher than that ofratio decreased patients. Conclusions We found that the CTL/Treg ratio of PBMC in metastaticcolorectal cancer patients was useful for the prediction of the effect of chemotherapy. |
