13C → 1H transfer of light-induced hyperpolarization allows for selective detection of protons in frozen photosynthetic reaction center
| Autor: | Karthick Babu Sai Sankar Gupta, Shimon Vega, A. Alia, Pavlo Bielytskyi, Kaustubh R. Mote, Daniel Gräsing, Jörg Matysik, Perunthiruthy K. Madhu |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
Photosynthetic reaction centre Nuclear and High Energy Physics biology Proton 010405 organic chemistry Chemistry Biophysics macromolecular substances 010402 general chemistry Condensed Matter Physics biology.organism_classification Photosynthesis Photochemistry 01 natural sciences Biochemistry Purple bacteria 0104 chemical sciences Rhodobacter sphaeroides Electron transfer Heteronuclear molecule Hyperpolarization (physics) |
| Zdroj: | Journal of Magnetic Resonance |
| ISSN: | 1090-7807 |
| Popis: | C labeled RCs from the purple bacteria of Rhodobacter sphaeroides. We observed response from the protons belonging to the photochemically active cofactors in their native protein environment. Such an approach is a potential heteronuclear spin-torch experiment which could be complementary to the classical heteronuclear correlation (HETCOR) experiments for mapping proton chemical shifts of photosynthetic cofactors and to understand the role of the proton pool around the electron donors in the electron transfer process occurring during photosynthesis. |
| Databáze: | OpenAIRE |
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