Redox-dependent activation of PI3-kinase is involved in growth-factor- induced proliferation of fibroblasts
| Autor: | Pyotr A. Tyurin-Kuzmin, G. D. Sagaradze, Alexander V. Vorotnikov, N. D. Zhdanovskaya, A. A. Sukhova |
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| Rok vydání: | 2017 |
| Předmět: |
inorganic chemicals
0301 basic medicine Platelet-derived growth factor biology Kinase Growth factor medicine.medical_treatment Biophysics Cell Biology Biochemistry Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Apocynin medicine biology.protein Phosphorylation Wound healing Protein kinase B Platelet-derived growth factor receptor |
| Zdroj: | Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology. 11:17-23 |
| ISSN: | 1990-7494 1990-7478 |
| DOI: | 10.1134/s199074781604022x |
| Popis: | Proliferation of fibroblasts is important for wound healing and reparation. Platelet-derived growth factor (PDGF) is a key regulator of this activity in fibroblasts. Activation of the PDGF receptor is also associated with endogenous generation of hydrogen peroxide (H2O2). Here we studied the involvement of H2O2 in the proliferative response of fibroblasts. We found that PDGF increased cytoplasmic level of H2O2. This increase was blocked by apocynin, an inhibitor of the membrane assembly of NADPH oxidases. Apocynin significantly decreased PDGF-stimulated phosphorylation of Akt and the mitotic activity of fibroblasts. Apocynin did not affect the activaion of the Erk1/2 MAP-kinase pathway. These data testify in favor of the H2O2 effect specificity. |
| Databáze: | OpenAIRE |
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