The effects of mutation and temperature variation on monacolin K production by monascus sp. and relative statistical parameter analysis of monacolin K production
| Autor: | Chung-Fu Huang, Wei-Ting Chen, Shu-Min Shen, Chih-Chun Chen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Mutation
Strain (chemistry) biology 010405 organic chemistry Chemistry Plant Science medicine.disease_cause Monascus biology.organism_classification 01 natural sciences Biochemistry 0104 chemical sciences 010404 medicinal & biomolecular chemistry Monascus sp Pigment visual_art Ethylmethane Sulfonate medicine visual_art.visual_art_medium Monacolin K Food science Agronomy and Crop Science Incubation Biotechnology |
| Zdroj: | Phytochemistry Letters. 32:143-150 |
| ISSN: | 1874-3900 |
| DOI: | 10.1016/j.phytol.2019.05.011 |
| Popis: | Monacolin K is a secondary metabolite of the Monascus species that restrains cholesterol synthesis. Temperature control is an effective method of improving the rate of monacolin K synthesis. Ultraviolet (UV) irradiation and ethylmethane sulfonate (EMS) were employed to mutate the Monascus strain so that it could be studied. After mutation by UV and EMS, the best monacolin K production strain was scanned after the 2 -h UV irradiation treatment. Monascus U-2 is defined as Monascus after UV irradiation for 2 h and generates the maximum production of monacolin K production. In the primary incubation period, monacolin K production of this strain was exceptionally low. Afterwards, cell growth reached the maximum value of 1.1 g/L after incubation for seven days. During the incubation process, the maximum production of monacolin K reached 8437.6 mg/kg by Monascus U-2, and the red pigment was 11.52. In summary, this study shows that the mutated strain U-2 can produce more monacolin K than the mutated strain U-10 and U-EMS. |
| Databáze: | OpenAIRE |
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