Some aspects of the selection of high-performance liquid chromatographic methods for the separation of chiral compounds in pharmaceutical analysis
| Autor: | K. Mihályfi, M. Gazdag, G. Szepesi |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
chemistry.chemical_classification
Chromatography Cyclodextrin Resolution (mass spectrometry) Organic Chemistry General Medicine Biochemistry Analytical Chemistry chemistry.chemical_compound Column chromatography chemistry Norgestrel medicine Methanol Enantiomer Acetonitrile Selectivity medicine.drug |
| Zdroj: | Journal of Chromatography A. 450:145-155 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(01)83900-5 |
| Popis: | The resolution of norgestrel enantiomers using cyclodextrin inclusion complexation was investigated. For the purity testing of levonorgestrel the separation method was optimized with regard to the simultaneous determination of enantiomeric and non-isomeric impurities at low concentrations. The dependence of the selectivity and efficiency of the separation on the nature and concentration of the organic solvent (methanol or acetonitrile) in the eluent and on column temperature was studied in order to establish the optimal conditions for the separation. The optimal method was validated and proved to be suitable for the determination as little as 0.1% of enantiomeric impurity in levonorgestrel. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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