Determination of Specific Class E Immunoglobulins to Bet v 1 Birch Allergen by the Immuno-PCR Method
| Autor: | T. I. Muravieva, M. A. Kostromina, Victor D. Pivovarov, Sergey K. Zavriev, Roman S. Esipov, M. A. Mokronosova, M. A. Simonova, S. V. Khlgatian, D. Yu. Ryazantsev |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Allergic reaction biology Chemistry Organic Chemistry medicine.disease_cause Immunoglobulin E Biochemistry Molecular biology law.invention 03 medical and health sciences Birch pollen 030104 developmental biology Allergen law medicine Recombinant DNA biology.protein Antibody Immuno pcr |
| Zdroj: | Russian Journal of Bioorganic Chemistry. 44:217-224 |
| ISSN: | 1608-330X 1068-1620 |
| DOI: | 10.1134/s1068162018010168 |
| Popis: | The aim of the work is the development of a method of detection of specific class E immunoglobulins (IgE) to the main Bet v 1 birch allergen based on immuno-PCR (iPCR). The recombinant Bet v 1 allergen was obtained in E. coli cells. Its ability to bind to specific IgE was confirmed by enzyme-linked immunosorbent assay (ELISA) using previously characterized sera of individuals with an allergic reaction to birch pollen and control sera in individuals, in which the reaction to this allergen is absent. Based on the obtained recombinant protein, the method of iPCR analysis of specific IgE to Bet v 1 was developed. It was demonstrated that iPCR sensitivity is comparable to ELISA sensitivity, and the titration curves of specific sera in iPCR (unlike those in ELISA) demonstrate a linear dependence; this makes the developed method preferable for quantitative estimation of specific IgE in sera as compared with ELISA. |
| Databáze: | OpenAIRE |
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