Association of the biocide 5-chloro-2-methyl-isothiazol-3-one with Pseudomonas aeruginosa and Pseudomonas fluorescens
| Autor: | Megan A. Diehl, John S. Chapman |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
biology
Chemistry Pseudomonas aeruginosa Pseudomonas chemical and pharmacologic phenomena Pseudomonas fluorescens Metabolism bacterial infections and mycoses biology.organism_classification medicine.disease_cause complex mixtures Microbiology Biomaterials Biochemistry Mechanism of action parasitic diseases Pseudomonadales medicine medicine.symptom therapeutics Waste Management and Disposal Bacteria Pseudomonadaceae |
| Zdroj: | International Biodeterioration & Biodegradation. 44:191-199 |
| ISSN: | 0964-8305 |
| DOI: | 10.1016/s0964-8305(99)00077-3 |
| Popis: | The biocide 5-chloro-2-methyl-isothiazol-3-one (CMI) associated rapidly with cells of Pseudomonas aeruginosa and Pseudomonas fluorescens with association being nearly complete within 10–15 min. The cells serve as a sink for CMI, concentrating it up to 400-fold. Kinetics of association are very similar amongst the strains examined. Examination of the relation of CMI concentration to the rate of association indicates that there are two kinetically distinguishable processes, with the breakpoint occurring around the transition from inhibitory to suprainhibitory levels of CMI. This suggests the rapid onset of toxic effects at suprainhibitory CMI concentrations affects the associative process. Discharging the proton motive force by treatment with uncoupling agents or selectively depleting it by treatment with inhibitors reduces the amount of CMI which associates with the cells. Selective depletion of the ATP pool has no effect. These results suggest that either the proton motive force (pmf) is involved directly in CMI association in an active transport process, or that an intact pmf is required for some facet of the cells metabolism which maintains the cells as a sink for CMI. The nonchlorinated analogue 2-methyl-isothiazol-3-one is a poor competitor for CMI association. |
| Databáze: | OpenAIRE |
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