Thioredoxin1 regulates conidia formation, hyphal growth, and trap formation in the nematode-trapping fungus Arthrobotrys oligospora
| Autor: | Shanzhuang Niu, Jian Tao, Heng Li, Ke-Qin Zhang, Ji’ai Wang, Zeng Weikun, Hua Yu, Xinglai Ji, Zhihong Hu, Weiwei Chen, Xiaowei Huang, Juan Li, Jun Li |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Hyphal growth 0303 health sciences biology 030306 microbiology Chemistry fungi Fungus biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Microbiology Nematophagous fungus Conidium 03 medical and health sciences Nematode 010608 biotechnology Thioredoxin Conidium formation Mycelium |
| Zdroj: | Annals of Microbiology. 69:1267-1274 |
| ISSN: | 1869-2044 1590-4261 |
| DOI: | 10.1007/s13213-019-01511-5 |
| Popis: | Arthrobotrys oligospora, a model nematophagous fungus that produces specific adhesive networks to capture nematodes, has been proposed as a potentially effective biological agent to control harmful plant-parasitic nematodes. Although thioredoxin has been characterized as playing important roles in many cellular processes in other species, its function in nematophagous fungi has not been studied. Here, the function of a thioredoxin homolog, Aotrx1, was investigated in A. oligospora. The encoding gene of Aotrx1 in the nematophagous fungus A. oligospora was knocked out by homologous recombination; strain growth was assessed. The ΔAotrx1 strain of A. oligospora showed a significant decrease in growth rate on different media (PDA, CMY, and TG), a 70% decrease of conidia production, and a lower germination rate compared with the wild type. The mutant strain was unable to form traps to capture nematodes and was more sensitive to SDS and H2O2. Thioredoxin is involved in conidia development, trap formation, normal mycelial growth, and resistance to environmental stresses in the nematode-trapping fungus A. oligospora. |
| Databáze: | OpenAIRE |
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