Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources
| Autor: | Enéas Gomes-Filho, O. B. De Oliveira-Neto, R. Da Rocha Fragoso, José Tarquinio Prisco, Juan Carlos Alvarez-Pizarro, M. F. GROSSI-de-SÁ |
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| Rok vydání: | 2014 |
| Předmět: |
inorganic chemicals
chemistry.chemical_classification biology Chemistry ATPase Inorganic chemistry food and beverages chemistry.chemical_element Plant Science Horticulture Nitrogen Hydrolysis chemistry.chemical_compound Enzyme Membrane biology.protein Vanadate Ammonium Potassium deficiency Nuclear chemistry |
| Zdroj: | Biologia plantarum. 58:507-514 |
| ISSN: | 1573-8264 0006-3134 |
| DOI: | 10.1007/s10535-014-0438-1 |
| Popis: | We studied the influence of inorganic nitrogen sources (NO3 − or NH4 +) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4 +-fed roots compared with NO3 − -fed roots. The optimum pH of the enzyme was slightly lower in NO3 − -fed roots than in NH4 +-fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 increased in roots grown under NH4 +. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3 − or NH4 +, but it was significantly reduced by vanadate in NH4 +-grown plants. |
| Databáze: | OpenAIRE |
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