Afifella aestuarii sp. nov., a phototrophic bacterium
| Autor: | Sailaja Buddhi, G. Suresh, Deepshikha Gupta, V Ramana Ch., Sasikala Ch. |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification biology Strain (chemistry) Afifella marina Alphaproteobacteria General Medicine Afifella pfennigii 16S ribosomal RNA biology.organism_classification 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences genomic DNA 030104 developmental biology chemistry Afifella Carotenoid Ecology Evolution Behavior and Systematics |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 70:327-333 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003756 |
| Popis: | An oval- to rod-shaped, motile, Gram-stain-negative, oxidase-positive, catalase-negative, pink-coloured phototrophic bacterium (designated as strain JA968T) was isolated from an estuary near Pata, Gujarat, India. Cells had an intracytoplasmic membrane architecture as lamellae and divided by budding. Strain JA968T had bacteriochlorophyll-a and spirilloxanthin series carotenoids as photosynthetic pigments. The strain exhibited photolithoautotrophic, photoorganoheterotrophic and chemoorganoheterotrophic growth modes and required thiamine as a growth factor. Strain JA968T had C18 : 1ω7c/C18 : 1ω6c as the predominant fatty acid with ubiquinone-10 (Q-10) and menaquinone-10 (MK-10) forming the quinone composition. The genomic DNA G+C content of the strain was 63.5 mol%. Pairwise comparison of 16S rRNA gene sequences showed that strain JA968T was highly similar to Afifella marina DSM 2698T (99.9 %) and Afifella pfennigii DSM 17143T (98.4 %). The average nucleotide identity values were 92 % between strain JA968T and A. marina DSM 2698T, and 78 % between strain JA968T and A. pfennigii DSM 17143T. The digital DNA-DNA hybridization values between strain JA968T and A. marina and A. pfennigii were 49 and 19 %, respectively. The genomic distinction was also supported by differences in phenotypic and chemotaxonomic characteristics. We propose that strain JA968T represents a new species of the genus Afifella with the name Afifella aestuarii sp. nov. The type strain is JA968T (=KCTC 15634T=NBRC 113338T). |
| Databáze: | OpenAIRE |
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