Production of cellulase byTrichoderma reeseifrom pretreated straw and furfural residues
| Autor: | Jianlong He, Xiaoyan Liu, Tong Zhan, Chun Hai Zhao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine integumentary system biology Filter paper General Chemical Engineering Substrate (chemistry) General Chemistry Cellulase Straw biology.organism_classification Furfural 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry 010608 biotechnology Yield (chemistry) biology.protein Lignin Food science Trichoderma reesei |
| Zdroj: | RSC Advances. 8:36233-36238 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c8ra05936e |
| Popis: | In this study, furfural residues were used as a substrate for cellulase production by the fungi Trichoderma reesei. The results indicated that a low pH and the presence of lignin in the furfural residues have an obvious impact on cellulase production by T. reesei. After pH adjustment, furfural residues could be used for cellulase production by T. reesei, with a higher filter paper activity (FPA) and a higher activity of CMCase compared to that yielded from furfural residues with pH unadjusted. After being washed with 1.6% (w/v) H2O2, all of the lignin in the furfural residues was removed, and an FPA of 7.1 FPU ml−1 and a CMCase activity of 3.4 IU ml−1 were obtained in 115 h, while pretreated straw could yield an FPA of 8.0 FPU ml−1 and a CMCase activity of 2.7 IU ml−1 in 160 h. Moreover, after being treated with H2O2, furfural residues could be used as an inducer in the production of cellulases. With the treated furfural residues added into the medium at the beginning of cultivation, T. reesei gave the maximum FPA (8.4 FPU ml−1) and CMCase activity (4.8 IU ml−1) at 142 h from pretreated straw, which is relatively high for cellulase production compared to that from most other agricultural wastes reported. |
| Databáze: | OpenAIRE |
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