Enhancing RNAi by using concatemerized double-stranded RNA
Autor: | Nallur Krishna Kumar, Gaddelapati Sharath Chandra, Ramaswamy Asokan, Maligeppagol Manamohan |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Diamondback moth biology viruses fungi Plutella RNA General Medicine biology.organism_classification 01 natural sciences Cell biology Lepidoptera genitalia 010602 entomology 03 medical and health sciences RNA silencing 030104 developmental biology RNA interference Insect Science Gene silencing Agronomy and Crop Science Gene |
Zdroj: | Pest Management Science. 75:506-514 |
ISSN: | 1526-498X |
DOI: | 10.1002/ps.5149 |
Popis: | Background RNA interference (RNAi) is a potential tool for functional characterization of genes and also in the management of insect pests. Accumulated literature reveals that the RNAi efficiency varies among insect species and is reported to be less efficient in lepidopteran insects. Results We attempted to enhance RNAi efficiency by concatemerizing short double-stranded RNA (dsRNA) sequence. Then the effectiveness of concatemerized dsRNAs (C-dsRNAs) was compared with non-concatemerized long dsRNA (NCL-dsRNA) in silencing of acetylcholinesterase (AChE) in the diamondback moth, Plutella xylostella (Lepidoptera), a major pest on cruciferous vegetables. Results revealed that the C-dsRNAs enhanced the RNAi efficiency in terms of higher target gene silencing and consequently resulted in lower larval weight gain and higher mortality compared to the NCL-dsRNA treatment. Even the lower concentration (0.5 µg) of C-dsRNAs had a relatively similar effect to that of higher concentrations (2 µg) of NCL-dsRNA. The enhanced RNAi efficiency with C-dsRNAs was plausibly due to the higher expression of core RNAi pathway genes, Dicer-2 and Argonaute-2 (Ago-2), which are known to govern the efficiency of RNAi. Conclusion Overall, C-dsRNA enhanced the RNAi activity over routinely used long dsRNA in P. xylostella and this strategy may be applied for effective management of insect pests. © 2018 Society of Chemical Industry. |
Databáze: | OpenAIRE |
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