66-P

Autor: Gizem Tumer, Robert A. Bray, Farris B. Alton, Tiffany K. Roberts-Wilson, Howard M. Gebel, Stuart N. Lauren
Rok vydání: 2013
Předmět:
Zdroj: Human Immunology. 74:98
ISSN: 0198-8859
DOI: 10.1016/j.humimm.2013.08.141
Popis: Aim To analyze the relationship between AMR, renal biopsy C4d staining and circulating DSA (MFI) levels. Methods Twenty four patients with confirmed DSAs and allograft biopsies were evaluated. HLA class I and II DSAs in sera collected at the time of biopsy were identified by a Luminex-based, Single Antigen Bead assay. C4d scoring was performed by immunohistochemistry. Patients with biopsy proven AMR were further classified by summing Banff scoring of peritubular capillaritis (ptc; scale of 0 to 3) and glomerulitis (g; scale of 0 to 3). Totals (ptc+g) were subdivided as mild (0-2), moderate (3, 4), and severe (5, 6). Results Seventeen patients were diagnosed with AMR: 10 experienced mild AMR, 3 moderate AMR and 4 severe AMR. All severe AMR patients possessed both HLA class I and class II DSAs (MFI range: 2600-24000). Of the 3 patients with moderate AMR, one had only class I, one had only class II, and one had both (MFI range: 3000-24000). Among patients with mild AMR 5 had class I only, 2 had class II only and the remaining 3 had both class I and class II DSAs (MFI range:1800-24000). AMR was not diagnosed in 7 DSA+ patients. Six of these patients only displayed class II DSAs (MFI range: 2200-25000). One patient had only class I DSA (MFI = 5100). [figure1] Conclusions This study demonstrates that the MFI levels of donor specific antibodies do not predict whether biopsies will be C4d positive or negative. Furthermore, MFI levels of DSAs are not predictive of the presence of AMR.
Databáze: OpenAIRE
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