Microfluidic systems for extracting nucleic acids for DNA and RNA analysis
| Autor: | Levent Yobas, Jing Li, Chew-Kiat Heng, Saxon Liw, Victor Samper, Tit Meng Lim, Yu Chen, Lin Cong, Hongmiao Ji, Wing Cheong Hui |
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| Rok vydání: | 2007 |
| Předmět: |
Lysis
Chemistry Metals and Alloys RNA Condensed Matter Physics Molecular biology DNA extraction Virus Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention chemistry.chemical_compound Biochemistry law Nucleic acid RNA extraction Electrical and Electronic Engineering Instrumentation DNA Polymerase chain reaction |
| Zdroj: | Sensors and Actuators A: Physical. 133:335-339 |
| ISSN: | 0924-4247 |
| DOI: | 10.1016/j.sna.2006.06.031 |
| Popis: | This paper is to review the differences in the developments of microfluidic chips for extracting genomic deoxyribonucleic acid (DNA) and viral ribonucleic acid (RNA) from blood by the Biosensor Focus Interest Group (BFIG) in Singapore. DNA was extracted in a multi-step process by isolating and lysing white blood cells (WBC), typically ∼10 μm in diameter. Viral RNA was extracted directly from the submicron viruses in the blood. In terms of basic microfluidic components required, both DNA and RNA extractions used similar mixers for mixing reagents, filters for capturing or separating the blood cells, and a binder for capturing and purifying the DNA/RNA molecules. The designs of the filters were adapted to either capture WBC for DNA isolation or capture all virus particles for RNA isolation. The designs of these two kinds of filters had to be different. Besides the differences in the sizes of WBC and viruses, the concentration of the virus particles is usually much lower than WBC. Thus, a much higher volume of blood for filtering would be required for extracting viral RNA, especially for the intention to detect the viruses at early onset of infection. With proper modifications of the protocols, it has been demonstrated that both genomics DNA and viral RNA could be extracted successfully in these microfluidic chips. The quality of the extracted samples was verified by polymerase chain reaction (PCR) and gel-electrophoresis after the extractions. |
| Databáze: | OpenAIRE |
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