Effect of post-prandial lipemia on canine biochemical parameters

Autor: Paula Lima de Oliveira, Natália Camila Minucci Bonatto, Beatriz Perez Floriano, Breno Fernando Martins de Almeida, Maria Rachel Melo Bosculo, Luiz Daniel de Barros
Rok vydání: 2020
Předmět:
Zdroj: Comparative Clinical Pathology. 29:763-775
ISSN: 1618-565X
1618-5641
Popis: Lipemia is one of the main causes of rejection of samples in veterinary clinical laboratories, interfering in biochemical determinations performed through spectrophotometry. However, it is not adequately known whether such interference causes clinical errors of interpretation in routine canine biochemical determinations, which is especially important when considering that lipemia cannot always be avoided in routine veterinary practice. This study assessed postprandial lipemia of healthy dogs fed with commercial feed, as well as the effect of lipemia induced in vitro by the addition of commercial lipid solution. Twenty healthy dogs were selected, for induction of postprandial lipemia, and the peak lipemic time (3 h after feeding) was compared with two non-lipemic times (12 h of fasting), 1 day before and 1 day after the induction of lipemia, respectively. Lipid solution was added to non-lipemic sera simulating triglyceride concentrations similar to the minimum, medium, and maximum values obtained at the lipemic time in vivo. Biochemical analyses were performed in semi-automated spectrophotometer using a set of commercial reagents. Variables were tested for normality and differences between the groups were tested using paired t test or Wilcoxon and ANOVA or Friedman tests. Both in vivo and in vitro lipemia caused significant biochemical changes in routine tests in healthy dogs, varying according to the analyte tested. Results from lipemic samples should be interpreted with caution for some determinations, whereas in some analyses, the need for fasting is dispensable.
Databáze: OpenAIRE
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