| Popis: |
Metabolism of radiolabeled arachidonic acid (∗AA) by blastocysts and endometrial slices recovered from five gilts 16 days after detection of estrus was studies in vitro. Blastocysts from each gilt were divided into four 216 ± 18 mg, and each portion was placed into a separate petri dish containing 15 ml modified minimum essential medium (MEM)_. The incubates from each gilt received either 25, 50, 100 or 200 μg radioinert arachidonic acid (AA). Endometrium was dissected from each uterin horn, sliced and duplicate 509 ± 3 mg portions from each gilt were placed into petri dishes containing 15 ml MEM and 200 μm AA. All incubates received 5 νCi of ∗AA (either [14C]-arichidonic acid or [3H]-arichidonic acid). The incubates were rocked at 37°C for 24 h in an atmosphere of 50% n2:45% O2:5% CO2. After incubation, tissues and MEM were separated by centrifugation. Metabolism of ∗AA was assessed in extracts of MEM and tissue homogenates by separating ∗AA and its metabolites on columns of Sephades LH-20. Blastocysts produced compounds that migrated with [3H]-13,14-dihydro-15-keto-PGF2α (∗PGFM), [3H]-PGE2 (∗PGE2) and [3H]-PGF2α (∗PGF2α). The greatest (P |
