| Autor: |
Cao Guangwen, Yang Guang, Chang Wen-jun, Hou Jian-guo, Sun Junwen, He Xiang, Tan Xiao-jie, Zhang HongWei, Timothy C. Thompson, Ma Liye |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Journal of Medical Colleges of PLA. 23:189-198 |
| ISSN: |
1000-1948 |
| DOI: |
10.1016/s1000-1948(08)60042-8 |
| Popis: |
Objective To elucidate distinct functions of a recently identified cancer metastasis-associated molecule, related to testes-specific, vespid, and pathogenesis protein-1 (RTVP-1) in the mammalian immune system. Methods Immunohistochemical assays and functional analysis on the immune system were performed on RTVP –/– mice and RTVP-1 +/+ mice. Protein-protein interaction (PPI) was used to predict the functions of RTVP-1. Results Abnormal lymphocyte growth kinetics and reduced numbers of CD8 + T cells were revealed in lymph nodes of RTVP-1 –/– mice. Expression of phenotypic markers of maturation in bone marrow-derived dendritic cells (DC) was impaired in RTVP-1 –/– DC following antigenic stimulation in vitro and RTVP-1 –/– DC failed to provide normal CD4 + T cell stimulatory activities in vivo . RTVP-1 –/– mice failed to generate normal CTL or antibody responses in vivo after vaccination. In vivo tumor challenge experiments using a mouse cancer cell line demonstrated that the growth rate of subcutaneous tumors in RTVP-1 –/– mice was significantly increased and CD8 + T cell infiltration significantly reduced compared to RTVP-1 +/+ mice. PPI showed that RTVP-1 protein closely interacted with molecules associated with immune response and cancer metastasis. Conclusion RTVP-1 might function as a tumor metastasis suppressor and immunosurveillance molecule in cancer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect