Soluble cell-bound and extracellular cyclodextrin glycosyltransferases ofBacillus macerans show identical enzymological characteristics and antigenicity

Autor:	Noémi Nógrády, Péter Boross, J. Fachet, Attila Szentirmai, István Pócsi, Éva Katona, Viktória Jeney, József Tözsér
Rok vydání:	1996
Předmět:	chemistry.chemical_classification Antigenicity Lysis Cyclodextrin General Medicine Cyclodextrin glycosyltransferase Biology Applied Microbiology and Biotechnology Cell membrane Enzyme medicine.anatomical_structure chemistry Biochemistry Glycosyltransferase Extracellular medicine biology.protein
Zdroj:	Journal of Basic Microbiology. 36:335-340
ISSN:	1521-4028 0233-111X
DOI:	10.1002/jobm.3620360507
Popis:	Soluble cell-bound and extracellular cyclodextrin glycosyltransferases of Bacillus macerans were purified to homogeneity using the subsequent steps of adsorption on starch, ion-exchange chromatography and gel filtration. The elution profiles and the relative molecular masses of the enzymes as well as their N-terminal sequences were found to be identical. Moreover, mouse monoclonal antibodies raised against the soluble cell-bound enzyme recognised equally well both the cell-bound and the extracellular enzyme preparations. Regarding that no signal peptide could be detected in either the cell-bound or the extracellular cyclodextrin glycosyltransferase the enzyme was probably entrapped between the cell membrane and the cell wall in the early stationary phase and was released during cell lysis without any modification.
Databáze:	OpenAIRE
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