Establishment of the optically pumped chemiluminescence technique for diagnostics
Autor: | M. Shintani, Y. Ichimori, T. Sugawara, K. Kondo, Marvin A. Motsenbocker, H. Masuya |
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Rok vydání: | 1993 |
Předmět: | |
Zdroj: | Analytical Chemistry. 65:403-408 |
ISSN: | 1520-6882 0003-2700 |
DOI: | 10.1021/ac00052a016 |
Popis: | A new chemiluminescence procedure was established that eliminates the need to use a separate oxidant such as hydrogen peroxide for chemiluminescence detection reactions. In the procedure called optically pumped chemiluminescence (OPC), reg light energy is used by a photosensitive dye to oxidize luminol and generate blue light. By pulsing the excitation light and measuring chemiluminescence during the off time, light scattering background was eliminated and sensitivity enhanced 10-fold |
Databáze: | OpenAIRE |
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