Establishment of the optically pumped chemiluminescence technique for diagnostics

Autor: M. Shintani, Y. Ichimori, T. Sugawara, K. Kondo, Marvin A. Motsenbocker, H. Masuya
Rok vydání: 1993
Předmět:
Zdroj: Analytical Chemistry. 65:403-408
ISSN: 1520-6882
0003-2700
DOI: 10.1021/ac00052a016
Popis: A new chemiluminescence procedure was established that eliminates the need to use a separate oxidant such as hydrogen peroxide for chemiluminescence detection reactions. In the procedure called optically pumped chemiluminescence (OPC), reg light energy is used by a photosensitive dye to oxidize luminol and generate blue light. By pulsing the excitation light and measuring chemiluminescence during the off time, light scattering background was eliminated and sensitivity enhanced 10-fold
Databáze: OpenAIRE