Nocardia tartaricans cells immobilized in sodium alginate–cellulose sulfate–poly (methylene-co-guanidine)capsules: mechanical resistance and operational stability
| Autor: | Alica Vikartovská, Ian W. Marison, Gabriela Kolláriková, Igor Lacík, Peter Gemeiner, Marek Bučko |
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| Rok vydání: | 2006 |
| Předmět: |
Chromatography
Renewable Energy Sustainability and the Environment Chemistry General Chemical Engineering Sodium Organic Chemistry Dispersity Size-exclusion chromatography chemistry.chemical_element Pollution Polyelectrolyte Inorganic Chemistry Hydrolysis Fuel Technology Membrane Biotransformation Hydrolase Waste Management and Disposal Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 81:500-504 |
| ISSN: | 1097-4660 0268-2575 |
| DOI: | 10.1002/jctb.1466 |
| Popis: | The stereospecific hydrolysis of cis-epoxysuccinate (CES) to L-(+)-tartrate, catalysed by the action of CES hydrolase activity in whole Nocardia tartaricans bacterial cells, has been chosen as a model biotransformation system. The performance of cells was studied by immobilization in polyelectrolyte complex (sodium alginate–cellulose sulfate–poly(methylene-co-guanidine, SA–CS/PMCG) capsules based on sodium alginate and cellulose sulfate as polyanions, poly(methylene-co-guanidine) as polycation, CaCl2 as gelling agent and NaCl as antigelling agent. The encapsulated cells showed a preservation of 80% of the initial catalytic activity in encapsulated cells compared with 15% of retained catalytic activity in free cells after 10 repeated biotransformation cycles. Uniformity of batches of capsules was achieved by the use of an air-stripping extrusion device fitted with a multiloop reactor. The capsules produced using this device had narrow standard deviations for capsule diameter ( |
| Databáze: | OpenAIRE |
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