Analysis of fast axial scanning scheme using temporal focusing with acousto-optic deflectors
| Autor: | Rui Du, Songchao Xue, Qingming Luo, Derong Li, Shaoqun Zeng, Kun Bi |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
business.industry media_common.quotation_subject Physics::Medical Physics Magnification Inertia Atomic and Molecular Physics and Optics Collimated light law.invention Lens (optics) Light source Optics law Group delay dispersion High spatial resolution Focal length business media_common |
| Zdroj: | Journal of Modern Optics. 56:81-84 |
| ISSN: | 1362-3044 0950-0340 |
| DOI: | 10.1080/09500340802499448 |
| Popis: | Multiphoton microscopy has gained broad application in biology and medicine in the recent decade by assessing biological structure and function with high spatial resolution. Recently it has achieved fast two-dimensional x–y imaging by using inertia-free scanning mechanisms. However, the axial scanning speed is severely limited by mechanical inertia. This paper proposes a fast axial scanning scheme using temporal focusing implemented with an inertia-free scanning device, such as an acousto-optic device (AOD), and presents an experimental demonstration of two-photon axial scanning over a 9 µm range using AODs operated from 80 to 120 MHz. A theoretical analysis provides the detailed characteristics of this method. The effects of both the ratio of collimating lens to objective lens focal lengths or magnification (M), and the degree of group delay dispersion (GDD) in the light source on the temporal focusing and axial scanning range are described by theory and experiments. |
| Databáze: | OpenAIRE |
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