| Popis: |
Expression of inborn immunity genes was studied in 10 essentially healthy male subjects aged 24 to 32 years in a 21-day dry immersion experiment without countermeasures. To evaluate the humoral immunity function, genes encoding Toll-like receptors, proteins mediating NF-kB, AP-1, Akt, TLR4; apoptosis proteins, and CLEC4E, HSPD1, PTGS2 were selected. Fenol-cloroform extraction was used to obtain total RNA from peripheral blood monocytes CD14+. Subsequently, total RNA was reverse transcribed to generate cDNA for real-time PCR. The results showed substantial changes in expression of these genes suggesting that changes in the TLRs function on the transcriptional level were at the very start of the immune response to 21-d DI. |
