Chromosome Painting Does Not Support a Sex Chromosome Turnover in Lacerta agilis Linnaeus, 1758
| Autor: | Massimo Giovannotti, Vladimir Trifonov, Artem P. Lisachov, Daria A. Andreyushkova, Jorge C. Pereira, Pavel M. Borodin, Svetlana A. Romanenko, Malcolm A. Ferguson-Smith |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Z chromosome biology Lizard Chromosome Karyotype biology.organism_classification 010603 evolutionary biology 01 natural sciences W chromosome 03 medical and health sciences Evolutionary biology Polymorphism (computer science) biology.animal Exigua Genetics Lacertidae Molecular Biology Genetics (clinical) 030304 developmental biology |
| Zdroj: | Cytogenetic and Genome Research. 160:134-140 |
| ISSN: | 1424-859X 1424-8581 |
| Popis: | Reptiles show a remarkable diversity of sex determination mechanisms and sex chromosome systems, derived from different autosomal pairs. The origin of the ZW sex chromosomes of Lacerta agilis, a widespread Eurasian lizard species, is a matter of discussion: is it a small macrochromosome from the 11-18 group common to all lacertids, or does this species have a unique ZW pair derived from the large chromosome 5? Using independent molecular cytogenetic methods, we investigated the karyotype of L. agilis exigua from Siberia, Russia, to identify the sex chromosomes. FISH with a flow-sorted chromosome painting probe derived from L. strigata and specific to chromosomes 13, 14, and Z confirmed that the Z chromosome of L. agilis is a small macrochromosome, the same as in L. strigata. FISH with the telomeric probe showed an extensive accumulation of the telomere-like repeat in the W chromosome in agreement with previous studies, excluding the possibility that the lineages of L. agilis studied in different works could have different sex chromosome systems due to a putative intra-species polymorphism. Our results reinforce the idea of the stability of the sex chromosomes and lack of evidence for sex-chromosome turnovers in known species of Lacertidae. |
| Databáze: | OpenAIRE |
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