MR 77 KDA factor derived from fibroblasts stimulates the invasion ability of breast-cancer cells
| Autor: | Katsumi Ikeda, Masakazu Yashiro, Kosei Hirakawa-Y.S. Chung, Tetsuro Ishikawa, Tadayoshi Hasuma, Tetsuji Sawada, Fumihiko Hato |
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| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty integumentary system biology business.industry Growth factor medicine.medical_treatment Mammary gland medicine.disease medicine.anatomical_structure Breast cancer Oncology Cancer research medicine biology.protein Conditioned medium Breast cancer cells Antibody business Breast carcinoma Fibroblast |
| Zdroj: | International Journal of Cancer. 92:181-186 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/1097-0215(200102)9999:9999<::aid-ijc1175>3.0.co;2-f |
| Popis: | Breast-cancer cells frequently invade into the skin. However, few reports have described the mechanisms responsible for this invasion. In this study, we investigated the effects of skin fibroblasts on the invasion ability of breast-cancer cells, using a modification of Boyden's chamber method, and purified an invasion-stimulating factor from fibroblasts. Conditioned medium of skin fibroblasts, DF-1, significantly stimulated the invasion ability of breast-cancer cells, OCUB-1. The invasion-stimulating factor produced from skin fibroblasts was then partially purified and characterized. The soluble invasion-stimulating factor of fibroblasts was a protein of 77 kDa, as calculated by SDS-PAGE. Although TGF-α and bFGF stimulated the invasion of OCUB-1, no inhibition of the 77 kDa factor was achieved with neutralizing antibodies against TGF-α and bFGF. These findings suggest that the 77 kDa factor derived from skin fibroblasts might be unique, might play an important role in invasion to the skin and might explain the frequent skin invasion in breast carcinoma. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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