Utility of the QIAGEN/artus PCR assays and Meningofinder™ for the detection of human herpes virus genomes in formalin-fixed paraffin-embedded clinical samples
| Autor: | Anna-Lena Hardt, Ramona-Liza Tillmann, Verena Schildgen, Oliver Schildgen, Michael Brockmann |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Future Virology. 9:241-242 |
| ISSN: | 1746-0808 1746-0794 |
| DOI: | 10.2217/fvl.14.7 |
| Popis: | ABSTRACT: Aim: The aim of the study was to analyze whether human herpes viruses can be detected from formalin-fixed paraffin-embedded (FFPE) tissues with two commercial methods. Materials & methods: In total, 91 clinical FFPE samples were analyzed with QIAGEN/artus (Hilden, Germany) qPCRs and the Meningofinder™ assay from PathoFinder (Maastricht, The Netherlands). Results: A total of 27 out of 91 samples were tested for HSV-1 and HSV-2. Three of these 27 samples (11.1%) were positive for HSV-1 and one sample was positive for HSV-2. Out of 91 samples, 50 were tested for CMV, of which six samples were positive (12%). In total, 12 out of 91 samples were tested for the presence of Varizella zoster virus DNA, revealing one as positive (8%). Finally, two samples were tested negative for EBV. Conclusion: Minor differences were identified between both assays, making them a reliable tool for detection of herpes viruses from FFPE material, although this is an off-label use for both assays. |
| Databáze: | OpenAIRE |
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