Adhesion ofDictyosteliumamoebae to deposited langmuir‐blodgett monolayers and derivatized solid surfaces. A study of the conditions which trigger a contact‐mediated cytoplasmic contractile response
| Autor: | D Gingell, N F Owens |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
biology
Stereochemistry Chemistry macromolecular substances Adhesion Aquatic Science biology.organism_classification Applied Microbiology and Biotechnology Dictyostelium Langmuir–Blodgett film Ionic strength Covalent bond Monolayer Biophysics Cell adhesion Water Science and Technology Lamella (cell biology) |
| Zdroj: | Biofouling. 5:205-226 |
| ISSN: | 1029-2454 0892-7014 |
| DOI: | 10.1080/08927019209378242 |
| Popis: | The motile behaviour of Dictyostelium amoebae on a variety of solid surfaces has been studied. These included deposited long chain alcohol, acid and glycoside monolayers, as well as glass derivatized with (a) covalent hydrophobic methyl groups (octadecyl glass) and amine groups (amine glass) and (b) an adsorbed polyamino acid. Amoebae spread on all these surfaces, but responded by strong centripetal cytoplasmic retraction, forming an ultrathin lamella on hydrophobic glass and amino glass. An extensive study of the cytoplasmic response on derivatized glass, relating the streaming (zeta) potential of the substratum to bulk pH and ionic strength, showed no simple correlation with the electrostatic properties of the interface. This response may be triggered when the strength of adhesion exceeds a certain threshold value. A tentative model for the transductive events linking cell adhesion to the cytoskeletal response is presented after a consideration of the motile machinery of Dictyostelium amoebae and its ac... |
| Databáze: | OpenAIRE |
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