Synthesis of propyl gallate by mycelium-bound tannase from Aspergillus niger in organic solvent

Autor:	Yong-Quan Li, Xiao-Wei Yu, Shi-Miao Zhou, Yu-Yi Zheng
Rok vydání:	2007
Předmět:	Tannase activity biology Physiology Aspergillus niger Alcohol General Medicine biology.organism_classification Applied Microbiology and Biotechnology Tannase Solvent chemistry.chemical_compound chemistry Sodium sulfate Organic chemistry Gallic acid Propyl gallate Biotechnology
Zdroj:	World Journal of Microbiology and Biotechnology. 23:1091-1098
ISSN:	1573-0972 0959-3993
DOI:	10.1007/s11274-006-9338-7
Popis:	Aspergillus niger with mycelium-bound tannase activity was employed to investigate the synthesis of propyl gallate from gallic acid and 1-propanol in organic solvents. The effects of various organic solvents (log P: −1.0 to 6.6) on the enzymatic reactions showed that benzene (log P: 2.0) was the most suitable solvent. The water content and protonation state of mycelium-bound enzyme both had significant effects on the activity of tannase. The maximum molar conversion (65%) was achieved with 7.3% (v/v) 1-propanol and 5.56 mM gallic acid at stirring speeds of 200 rev/min, 40 °C in presence of anhydrous sodium sulfate and PEG-10,000. Enzyme specificity for the alcohol portion (C1–C8) of the ester showed that the optimum synthesis was observed with alcohols ranging from C3 to C5.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4391f52606f00e18a155766af3a3d21a https://doi.org/10.1007/s11274-006-9338-7 Zobrazit plný text záznamu Full text from SpringerLink